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高致病性EV71毒株的感染易伴发神经系统症状,是导致手足口病死亡病例的主要原因,但目前临床上仍缺乏防治EV71感染的特效药物及安全有效的疫苗。为进一步明确EV71的致病机制,本项工作以高致病性的EV71-HP临床分离株及其经体外细胞培养适应的EV71-CCA毒株的基因组RNA为模板,应用反向遗传学方法构建了二者的感染性克隆。研究表明HP株在细胞中的增殖速度明显快于CCA株。两株基于感染性克隆的拯救病毒分别保持了与各自母本株基因组序列的一致性,同时也保留了母本株增殖表型的差异,包括病毒的增殖速度以及病毒的温度敏感性。本工作将促进对EV71的致病机制以及对EV71疫苗的研究。
The infection of highly pathogenic EV71 strains is easily accompanied by neurological symptoms, which is the main cause of hand-foot-mouth disease death. However, there is still no effective drug for preventing and treating EV71 infection and a safe and effective vaccine. In order to further clarify the pathogenesis of EV71, this work constructed a retroviral-based approach using genomic RNA of highly pathogenic EV71-HP clinical isolates and their in vitro cell culture-adapted EV71-CCA strains as templates The two infectious clones. Studies have shown that HP strains proliferate significantly faster in cells than CCA strains. The two infectious viruses based on infectious clones, respectively, retained the same sequence as the genomic sequences of their respective maternal strains, while preserving the differences in the proliferative phenotypes of the maternal strains, including the rate of virus proliferation and the temperature sensitivity of the virus. This work will promote the pathogenesis of EV71 and EV71 vaccine research.