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背景:颞叶皮质与人类和动物的学习记忆能力有关,它不仅接受听觉的投射纤维,还与许多空间认知相关的脑区有密切关系。目的:观察单侧颞叶皮质脑梗死大鼠经溶栓胶囊治疗后空间认识能力的改善情况。设计:完全随机对照实验。单位:解放军第一七五医院神经内科,解放军总医院神经内科及中国科学院心理研究所。材料:实验于1998-08/1999-02在中国科学院心理研究所脑功能研究室完成,选择SD大鼠28只,随机分为3组:脑梗死组10只,治疗组10只,假手术组8只。方法:立体定向光化学诱导制作脑梗死大鼠模型。水合氯醛麻醉,尾静脉注射玫瑰红溶液20mg/kg,左侧颞部切开头皮,暴露颞骨,光导纤维引导卤素灯冷光源,定向照射20min,面积2mm2。照射20min后治疗组腹腔注射溶栓胶囊生理盐水溶液(40g/L)200mg/kg,脑梗死组腹腔内注射等量生理盐水。假手术组尾静脉及腹腔注射生理盐水外,手术照射条件相同。主要观察指标:各组大鼠在Morris水迷宫中的训练成绩,包括:①反应时。②搜索策略(分为边缘式记1分,随机式记2分,趋向式记3分,直线式记4分)。结果:28只大鼠均进入结果分析。①各组大鼠平均反应时比较:治疗组和假手术组短于脑梗死组犤第1天:58,50,65s;第2天:24,27,46s;第3天:14,17,20s;第4天:11,9,15s,(P<0.01)犦,治疗组与假手术组基本接近(P>0.05)。②搜索策略评分:第1天可见假手术组及治疗组稍高于脑梗死组,但不稳定(F=1.167,P>0.05),第2天假手术组高于脑梗死组犤2.9,2.3分,(F=5.5,P<0.05)犦,但治疗组与脑梗死组基本接近犤2.6,2.3分,(F=3.34,P>0.05)犦,第3,4天治疗组均高于脑梗死组犤3.5,2.7分;3.7,3.3分,(F=8.92,14,P<0.01)犦。治疗组与假手术组基本接近(P>0.05)。结论:脑梗死早期给予溶栓胶囊治疗后,反应时明显缩短,搜索策略逐渐由边缘式和随机式过渡到趋向式和直线式,说明溶栓胶囊可改善脑缺血后的认知功能障碍,提高脑梗死大鼠的训练成绩。
Background: The temporal lobe cortex is associated with learning and memory abilities in humans and animals. It not only receives auditory projection fibers but is also closely related to many spatial cognition-related brain regions. OBJECTIVE: To observe the improvement of spatial cognitive ability after thrombolysis capsule treatment in unilateral temporal cortex cerebral infarction. Design: Complete randomized controlled experiment. Unit: Department of Neurology, No.175 Hospital of Chinese PLA, Department of Neurology, PLA General Hospital and Institute of Psychology, Chinese Academy of Sciences. MATERIALS: Experiments were performed in the Brain Function Laboratory, Institute of Psychology, Chinese Academy of Sciences from August 1998 to February 1999. Twenty-eight SD rats were randomly divided into three groups: 10 in the infarction group, 10 in the treatment group, 8 only Methods: stereotactic photochemical induction of cerebral infarction rat model. Chloral hydrate anesthesia, tail vein injection rose red solution 20mg / kg, the left temporal incision scalp exposed temporal bone, optical fiber guide halogen cold light source, directional irradiation 20min, an area of 2mm2. The rats in the treatment group were injected intraperitoneally with 200mg / kg of thrombolytic capsule (40g / L) 20min after irradiation. The rats in the cerebral infarction group were given the same amount of normal saline by intraperitoneal injection. Sham-operated group tail vein and intraperitoneal injection of saline, the same surgical irradiation conditions. MAIN OUTCOME MEASURES: The training results of rats in Morris water maze, including: ① reaction time. ② search strategy (divided into the edge of a record, random record 2 points, trend record 3 points, straight record 4 points). Results: All 28 rats were involved in the result analysis. ①The average reaction time of rats in each group was shorter than that in the control group and the sham group (P <0.05). The first day: 58,50,65s; the second day: 24,27,46s; the third day: 14,17, 20s; on the 4th day: 11, 9, 15s, (P <0.01) 犦, the treatment group and sham operation group were basically close (P> 0.05). (2) Search strategy score: On the first day, the sham group and the treatment group were slightly higher than those in the cerebral infarction group, but unstable (F = 1.167, P> 0.05). On the second day, the sham operation group was higher than the cerebral infarction group (F = 3.34, P> 0.05) 犦, the treatment group were higher than the brain on the 3rd and the 3rd days in the treatment group and the cerebral infarction group (P <0.05) Infarct 犤 3.5,2.7 points; 3.7,3.3 points (F = 8.92,14, P <0.01) 犦. The treatment group and the sham group were basically close (P> 0.05). CONCLUSION: After the treatment of thrombolytic capsules in the early stage of cerebral infarction, the response time is obviously shortened. The search strategy gradually transitions from marginal and random to the trend and straight line, indicating that thrombolytic capsule can improve the cognitive dysfunction after cerebral ischemia, Improve the training results of cerebral infarction rats.