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AIM:To study the reversing effect of Ginkgo biloba extract(GbE) on established liver fibrosis in rats.METHODS:Following confirmation of CCl_4-induced liverfibrosis,GbE or saline was administrated to the rats for4 weeks.The remaining rats received neither CCl_4 norGbE as normal control.The four groups were compared interms of serum enzymes,tissue damage,expression ofαSMA and tissue inhibitor-1 of metalloproteinase (TIMP-1)and metalloproteinase-1 (MMP-1).RESULTS:Compared with saline-treated group,liverfibrosis rats treated with GbE had decreased serum totalbilirubin (P<0.01) and aminotransferase levels (P<0.01)and increased levels of serum albumin (P<0.01).Microscopicstudies revealed that the livers of rats receiving GbE showedallieviation in fibrosis (P<0.05) as well as expression ofαSMA (P<0.01).The liver collagen and reticulum contentswere lower in rats treated with GbE than saline-treatedgroup (P<0.01).RT-PCR revealed that the level of TIMP-1decreased while the level of MMP-1 increased in GbE group.CONCLUSION:Administration of GbE improved CCl_4-inducedliver fibrosis.It is possibly attributed to its effect of inhibitingthe expression of TIMP-1 and promoting the apoptosis ofhepatic stellate cells.
AIM: To study the reversing effect of Ginkgo biloba extract (GbE) on established liver fibrosis in rats. METHODS: Following confirmation of CCl_4-induced liver fibrosis, GbE or saline was administrated to the rats for 4 weeks. The remaining rats received neither CCl_4 norGbE as normal control. The four groups were compared intercellular of serum enzymes, tissue damage, expression of α SMA and tissue inhibitor-1 of metalloproteinase-1 (MMP-1) .RESULTS: Compared with saline-treated group, liverfibrosis Rats were treated with GbE had decreased serum total bilirubin (P <0.01) and aminotransferase levels (P <0.01) and increased levels of serum albumin (P <0.01) .Microscopicstudies revealed that the livers of rats received GbE showed annexin in fibrosis The liver collagen and reticulum contents were lower in rats treated with GbE than saline-treatedgroup (P <0.01). RT-PCR revealed that the level of TIMP-1 decreased while the level of MMP- 1 incre ased in GbE group. CONCLUSION: Administration of GbE improved CCl_4-inducedliver fibrosis. It is possibly attributed to its effect of inhibiting the expression of TIMP-1 and promoting the apoptosis of hepatic stellate cells.