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目的探讨CO2气腹对胆囊癌细胞抗凋亡能力的影响。方法建立腹腔镜CO2气腹体外模型,以12mmHg(1mmHg=0.133kPa)的CO2气腹气压对胆囊癌细胞进行不同时间(1,2,3,4h)的处理。设未处理为对照组。采用RT-PCR检测CO2气腹处理后各组细胞survivin mRNA的表达情况。结果当压力为12mmHg时,survivin的表达随气腹处理时间的延长而呈上升趋势(P<0.05)。结论在CO2气腹(12mmHg)下作用3～4h能提高胆囊癌细胞的抗凋亡能力。这可能是CO2气腹促肿瘤生长的机制之一。
Objective To investigate the effect of CO2 pneumoperitoneum on apoptosis of gallbladder carcinoma cells. Methods The model of laparoscopic CO2 pneumoperitoneum was established. The gallbladder carcinoma cells were treated with CO2 pneumoperitoneum pressure of 12mmHg (1mmHg = 0.133kPa) for different time (1,2,3,4 h). Set untreated control group. The expression of survivin mRNA in each group was detected by RT-PCR after CO2 pneumoperitoneum treatment. Results When the pressure was 12mmHg, the expression of survivin increased with the time of pneumoperitoneum treatment (P <0.05). Conclusion CO2 pneumoperitoneum (12mmHg) for 3 ~ 4h can improve the anti-apoptotic ability of gallbladder carcinoma cells. This may be CO2 pneumoperitoneum one of the mechanisms of tumor growth.