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目的 分析不同病变胃黏膜细胞内端粒长度的差异 ,以及细胞内DNA含量 ,探讨端粒行为异常、细胞内DNA含量与胃黏膜癌变的关系。方法 应用Southern杂交分析细胞内端粒长度 ,应用流式细胞术测定细胞内DNA含量。结果 在 172例胃镜活检标本中 ,正常胃黏膜 ,慢性浅表性胃炎(CSG) ,伴 0、1、2度肠化的慢性萎缩性胃炎 (CAG)和胃癌组织的端粒长度 ,分别是 (10 .42± 0 .2 )kb ,(9 .86± 0 .4)kb ,(9.78± 1.2 )kb、(8.6 4± 1.0 )kb、(6 .2 2± 1.2 )kb和 (5 .86± 2 .6 )kb。 45例胃癌手术标本结果相似。流式细胞术分析细胞内DNA含量 ,在胃镜活检标本中 ,正常胃黏膜 ,CSG ,伴 0、1、2度肠化的CAG和胃癌组织的异倍体DNA检出率分别为 0、0、0、10 .0 0 %、12 .5 0 %和 33 .33%。45例胃癌手术切除标本结果相似。异倍体细胞内端粒长度明显短于二倍体细胞 ,异倍体细胞中端粒长度与DNA指数呈负相关 (r=- 0 .91,P <0 .0 1) ,即端粒越短 ,DNA指数越高。结论 端粒长度从正常胃黏膜、不同程度肠化胃黏膜到癌变胃黏膜逐渐缩短。在正常胃黏膜和CSG中未检出异倍体DNA ,从 1度、2度肠化到癌变胃黏膜异倍体DNA检出率逐渐增高 ,而在异倍体细胞中端粒长度和DNA指数呈负相关。推测 ,可能存在端粒愈短 ,DNA扩增愈活跃。端粒缩短?
Objective To analyze the difference of telomere length in different lesions of gastric mucosa and the intracellular DNA content, and to investigate the relationship between abnormal telomere behavior, intracellular DNA content and gastric carcinogenesis. Methods The telomere length in cells was analyzed by Southern blotting. The content of DNA in cells was determined by flow cytometry. Results In 172 endoscopic biopsy specimens, the telomere lengths of normal gastric mucosa, chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG) with 0, 1 and 2 degrees of intestinal metaplasia, and gastric cancer tissue were ( 10.42±0.2 kb, (9.86±0.4) kb, (9.78±1.2) kb, (8.6 4±1.0) kb, (6.22±1.2) kb, and (5.86) ± 2 .6 ) kb. The results of 45 gastric cancer surgical specimens were similar. The intracellular DNA content was analyzed by flow cytometry. In gastroscope biopsy specimens, the detection rates of normal gastric mucosa, CSG, CAG with 0, 1 and 2 degrees of intestinal metaplasia, and gastric cancer tissues were 0, 0, respectively. 0,10.0%, 12.5%, and 33.33%. 45 cases of surgical resection of gastric cancer were similar. The length of telomere in aneuploid cells was significantly shorter than that of diploid cells, and the telomere length was negatively correlated with the DNA index in aneuploid cells (r = -0.91, P < 0.01). Shorter, the higher the DNA index. Conclusion The telomere length is gradually shortened from normal gastric mucosa, varying degree of intestinal metaplasia to cancerous gastric mucosa. Aneuploid DNA was not detected in normal gastric mucosa and CSG, and the detection rate of aneuploid DNA in gastric mucosa from 1 degree and 2 degrees of intestinal metaplasia was gradually increased, while the telomere length and DNA index in aneuploid cells were increased. Negative correlation. Presumably, there may be shorter telomeres and more vigorous DNA amplification. Telomere shortening?