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检测生理体液及组织中多胺水平可以做为临床辅助诊断、治疗癌症,评价抗癌药物的药效及监视术后化疗的愈后效果指标。本文报道利用岛津LC-3A型高效液相色谱法定量分析生理体液及组织中腐胺、精脒和精胺具有操作简便、快速、灵敏和特异性强等优点。色谱分析条件:岛津LC-3A型高效液相色谱仪,配备SPD-1紫外检测器242nm波长和C-RIB色谱数据处理机。分析柱:YQG-C18(250×4.6mm
Detection of physiological fluid and tissue polyamines levels can be used as a clinical diagnosis, treatment of cancer, evaluate the efficacy of anti-cancer drugs and monitor postoperative chemotherapy prognostic indicators. This paper reports the use of Shimadzu LC-3A-based high performance liquid chromatography quantitative analysis of physiological fluids and tissues of putrescine, spermine and spermine has the advantages of simple, rapid, sensitive and specific advantages. Chromatographic conditions: Shimadzu LC-3A high performance liquid chromatograph equipped with SPD-1 UV detector 242nm wavelength and C-RIB chromatographic data processor. Analytical column: YQG-C18 (250 × 4.6 mm