The tobacco growers with the growing area of greater than or equal to 100 mu,have become an important subject of tobacco production in Weng’an County of Guizhou Province. Regulating and developing the production and business activities of large-scale tobacco growers, plays an important role in stabilizing tobacco production,reducing costs and increasing efficiency in Weng’an County. Through the field survey of large-scale growers’ production activities,this article analyzes the input and output levels,and explores the key factors influencing benefit, in order to provide a basis for further regulating the tobacco growing practices and improving the flue-cured tobacco production benefit. The tobacco growers with the growing area of ​​greater than or equal to 100 mu, have become an important subject of tobacco production in Weng’an County of Guizhou Province. Regulating and developing the production and business activities of large-scale tobacco growers, plays an important role in stabilizing tobacco production, reducing costs and increasing efficiency in Weng’an County. Through the field survey of large-scale growers’ production activities, this article analyzes the input and output levels, and explores the key factors influencing benefit, in order to provide a basis for further regulating the tobacco growing practices and improving the flue-cured tobacco production benefit.