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经逆转录病毒载体将人GM-CSF基因导入人膀胱癌细胞株BIU-87细胞中,建立了转基因细胞株BIU/GM。经流式细胞仪行细胞DNA周期分析表明GM-CSF基因的导人及表达对BIU-87细胞的增长无影响。免疫荧光测定发现转GM-CSF基因及表达不能促进BIU-87细胞表面HLA-ABC、DR、DQ抗原的表达。转基因瘤细胞株经6000rad X射线照射灭活后,丧失增殖能力,逐步死亡,但能维持一定水平的GM-CSF分泌活性达两周以上。从而为制备灭活的转基因瘤苗提供了初步经验。
The human GM-CSF gene was introduced into human bladder cancer cell line BIU-87 cells by retroviral vector, and the transgenic cell line BIU / GM was established. Cell cycle analysis by flow cytometry showed that the induction and expression of GM-CSF gene had no effect on the growth of BIU-87 cells. Immunofluorescence assay showed that transfection of GM-CSF gene and expression could not promote the expression of HLA-ABC, DR and DQ antigens on BIU-87 cells. After inactivated by 6000 rad X-ray, the transgenic tumor cell lines lost their ability to proliferate and died gradually, but maintained a certain level of GM-CSF secretion for more than two weeks. Thus providing preliminary experience for preparing inactivated transgenic tumor vaccine.