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目的探索黄芪等中药调节机体免疫功能的物质基础及其部分机制。方法提取黄芪、枸杞、香菇、地黄、防风、当归、柴胡、板蓝根、蒲公英等9味中药多糖组分,分大、中、小剂量灌胃干预实验小鼠,连续给药7d后,采用ELISA法检测实验动物血清中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)等细胞因子水平,并与空白对照组比较。结果 1黄芪、枸杞、地黄等3味中药多糖组分一定浓度组小鼠血清GM-CSF、G-CSF、M-CSF中一个或多个集落刺激因子较空白对照组显著升高(P<0.05或P<0.01),且具有浓度差异性(P<0.05或P<0.01);2当归多糖组分各浓度组小鼠血清M-CSF水平较空白对照组显著减低(P<0.01),且具有浓度差异性(P<0.01)。结论黄芪、枸杞、地黄、当归等4味中药的多糖组分对机体集落刺激因子的干预效应的可能是这些中药免疫调节作用的部分途径。
Objective To explore the material basis and some mechanisms of astragalus and other traditional Chinese medicines for regulating immune function. Methods Nine polysaccharides from Radix astragali, Chinese wolfberry, shiitake mushroom, Rehmannia glutinosa, Radix et Rhizoma Rhei, Radix Angelicae Sinensis, Radix Bupleurum, Radix Isatidis and dandelion were fractionated into experimental groups by intragastric administration of large, medium and small doses. Methods The levels of cytokines such as GM-CSF, G-CSF and M-CSF in serum of experimental animals were detected and compared with Blank control group comparison. Results 1 Astragalus membranaceus, Lycium barbarum, Rehmannia glutinosa and other three kinds of Chinese medicine polysaccharide components A certain concentration of serum GM-CSF, G-CSF, M-CSF in one or more colony stimulating factor than the blank control group was significantly increased (P <0.05 (P <0.05 or P <0.01) .2 The level of serum M-CSF in each concentration of Angelica Sinensis polysaccharide group was significantly lower than that of the blank control group (P <0.01) Concentration difference (P <0.01). Conclusion The intervention effects of polysaccharides components such as Astragalus membranaceus, Lycium barbarum, Radix et Rhizoma Angelica and Angelica sinensis on the colony-stimulating factor may be part of the immunoregulatory effects of these traditional Chinese medicines.