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选择Wister大鼠127只,用放射免疫分析法、黑质(NS)微量注射及荧光分光光度测定法,探讨NS内神经降压素(NT)与多巴胺(DA)的相互作用及机制。结果表明,1.NS内微量注射不同浓度的NT后,NS内的DA含量较对照组明显增加(P<0.01),且呈明显的量效依赖关系(P<0.05);2.NS内注射异搏定后,NS内的DA含量较对照组明显减少(P<0.05);3.NS、第三脑室和下丘脑分别注射抗NT血清后,NS内的DA含量与对照组相比无明显变化(P>0.05);4.腹腔注射不同浓度的L-多巴和苄丝肼后,NS内的NT免疫活性物含量较对照组明显增加(P<0.01),且呈明显的量效依赖关系(P<0.01);5.腹腔注射氟哌啶醇后,再注射L-多巴和苄丝肼,NS内的NT免疫活性物含量与对照组相比无明显变化(P>0.05)。提示外源性NT能促进NS内DA的释放,这可能与Ca2+有关,而内源性NT不参与NS内DA的释放过程;NS内的DA能促进其NT的释放,此效应与DA-D2受体无关。
127 Wister rats were selected to investigate the interaction and mechanism of neurotensin (NT) with dopamine (DA) in NS by radioimmunoassay, microinjection of NS and fluorescence spectrophotometry. The result shows that: 1. After intradermic injection of NT with different concentrations, the content of DA in NS increased significantly (P <0.01) compared with that in control group (P <0.05) .2. After injection of verapamil into NS, the content of DA in NS decreased significantly compared with that in control group (P <0.05) .3. NS, third ventricle and hypothalamus were injected anti-NT serum, NS content in DA compared with the control group had no significant change (P> 0.05); After intraperitoneal injection of L-dopa and benserazide at different concentrations, the content of NT immunoreactive substance in NS increased significantly (P <0.01) compared with the control group, and showed a significant dose-dependent relationship (P <0.01 5) After intraperitoneal injection of haloperidol, L-dopa and benserazide were injected again. The content of NT immunoreactive substance in NS had no significant difference compared with the control group (P> 0.05). It is suggested that exogenous NT can promote the release of DA in NS, which may be related to Ca2 +, whereas endogenous NT is not involved in the release of DA in NS. The intranasal DA can promote the release of NT, which is similar to DA-D2 Receptor has nothing to do.