论文部分内容阅读
本实验采用洗涤人血小板和人脐动脉环孵育技术,观察二十碳五烯酸(EPA,20:5n3)和二十二碳六烯酸(DHA,22:6n3)对外源性标记花生四烯酸(AA,20:4n6)在血小板和动脉壁内代谢转化的影响。结果表明,EPA和DHA对血小板中血栓素B_2的生成和动脉壁6-Keto-PGF_(1a)的生成均产生剂量依赖性抑制。EPA在低浓度(62.5μM)时可引起血小板生成脂氧化酶产物HETE增加,随EPA浓度升高,HETE产量的增加率降低,甚至在高浓度(1000μM)时表现出HETE生成的抑制,但不显著。DHA除在62.5μM这一浓度时使HETE增加不显著外,其余浓度则以剂量依赖式显著增加HETE的生成。本文结合先前的有关研究资料,就本实验结果进行了讨论。
In this study, the platelet-washing and human umbilical artery ring incubation techniques were used to observe the effect of eicosapentaenoic acid (EPA, 20: 5n3) and docosahexaenoic acid (DHA, 22: 6n3) Effect of Acid (AA, 20: 4n6) Metabolic Transformation in Platelets and Arterial Walls. The results showed that EPA and DHA produced a dose-dependent inhibition of thromboxane B 2 production and arterial wall 6-Keto-PGF_ (1a) production in platelets. EPA, at low concentrations (62.5 [mu] M), caused an increase in platelet-forming lipoxygenase product HETE with a decrease in the rate of HETE production as EPA concentration increased, but showed an inhibition of HETE production even at high concentrations (1000 [mu] M) Significant. DHA, except that HETE increased insignificantly at a concentration of 62.5 [mu] M, the remaining concentrations significantly increased HETE production in a dose-dependent manner. In this paper, the previous relevant research data, the experimental results were discussed.