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目的 探讨乙型肝炎病毒 (HBV)DNA整合与p16的关系及其在肝细胞癌发生发展中的作用。方法 聚合酶链式反应 (PCR )检测 3 5例肝癌、癌旁组织及 3 1例肝硬化组织中HBVDNA及其整合情况 ,以Southernblot证实 ;逆转录 聚合酶链式 (RT PCR)检测组织中 p16mRNA。结果 有HBVDNA整合的肝癌、癌旁及肝硬化组织中X基因整合率分别为 10 0 .0 %、81.8%及68.4% ,三者差异有显著性 (P <0 .0 5 ) ;p16mRNA的表达缺失率分别为 5 1.4%、3 1.4%、2 2 .6% ,三者差异有显著性 (P <0 .0 5 )。结论 HBVDNA整合与 p16基因表达改变有相关性 ,两者在肝癌的发生发展中发挥重要作用。
Objective To investigate the relationship between hepatitis B virus (HBV) DNA integration and p16 and its role in the development of hepatocellular carcinoma. Methods The HBV DNA and its integration in 35 hepatocellular carcinoma tissues and 31 cirrhotic tissues were detected by polymerase chain reaction (PCR) and confirmed by Southern blot. The expression of p16 mRNA in tissues was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) . Results The integration rates of X gene in HBVDNA-integrated hepatocarcinoma, paracarcinoma and cirrhosis tissues were 100.0%, 81.8% and 68.4%, respectively (P <0.05), and the expression of p16 mRNA was absent Rates were 5 1.4%, 3 1.4%, 22.6%, the difference was significant (P <0. 05). Conclusion The integration of HBVDNA is related to the change of p16 gene expression, both of which play an important role in the occurrence and development of hepatocellular carcinoma.