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目的 探讨氟对大鼠脑细胞DNA的损伤作用及诱导凋亡的作用。方法 体内试验中 ,对照组大鼠腹腔注射蒸馏水 ,氟组大鼠注射氟化钠 ,染毒剂量为 2 0mg·kg- 1 ·d- 1 ;体外试验中 ,采用 5mmol/L氟化钠对急性分离的大脑神经细胞染毒 2h后用单细胞凝胶电泳技术 (SCGE)研究氟对细胞DNA的损伤 (单链断裂 )。采用TUNEL法和流式细胞术 (FCM)检测细胞凋亡。结果 体内试验氟组大鼠大脑皮层神经细胞DNA损伤情况明显比对照组严重 ,Ridit值分别为 0 351和 0 639,体外试验对照组与氟组Ridit值分别为 0 650 1和 0 3844。TUNEL阳性细胞在氟组大鼠大脑皮层、海马及小脑颗粒细胞层均可见到 ,在对照组大鼠则很罕见。流式细胞术检测结果表明大脑皮层及海马神经细胞凋亡百分率氟组 (2 7 1 2± 3 0 8,34 97± 5 46)均高于对照组 (4 63± 0 98,5 35± 0 79) ,差异有极显著性意义。结论 氟化钠可引起大鼠脑细胞DNA损伤和细胞凋亡发生
Objective To investigate the effect of fluoride on DNA damage and apoptosis in rat brain cells. Methods In vivo, the rats in the control group were injected intraperitoneally with distilled water and the rats in the fluoride group were injected with sodium fluoride at a dose of 20 mg · kg -1 · d -1. In vitro experiments, 5 mmol / L sodium fluoride Isolated brain neurons were exposed to Fluorine for DNA damage (single-strand breaks) by single cell gel electrophoresis (SCGE) 2 hours after exposure. Apoptosis was detected by TUNEL method and flow cytometry (FCM). Results In vivo, the DNA damage of neurons in cerebral cortex of rats in fluoride group was significantly worse than that in control group. Ridit values were 0 351 and 0 639, respectively. The Ridit values of control group and fluoride group in vitro were 0 650 1 and 0 3844, respectively. TUNEL-positive cells were observed in the cerebral cortex, hippocampus and cerebellar granule cell layer in the fluoride group, but rare in the control group. Flow cytometry results showed that the percentages of apoptotic neurons in the cerebral cortex and hippocampus were higher than those in the control group (2 7 1 2 ± 3 0 8, 34 97 ± 5 46) (4 63 ± 0 98, 5 35 ± 0 79), the difference is extremely significant. Conclusion Sodium fluoride can cause DNA damage and apoptosis in rat brain cells