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用2.25%颗粒状琼脂溶液混合、加热使液体澄清,用磁棒轻轻搅拌释出气体。取长方形试纸,在一端距4mm处作一标记,用镊子夹起试纸,使标记下端短暂浸泡入琼脂内,提起冷却、空气内干燥几秒钟。如此反复2～4次,直到试纸上形成约3mm厚的琼脂板层。将其在空气中干燥5～10分钟,然后贮存于50%乙醇液中。应用前,用无菌棉签或海绵轻擦琼脂板层。视网膜活检组织标本取出后,立即置于琼脂条上;或将标本浸入培养皿内平衡液中,
2.25% granular agar solution mixed, heated to clarify the liquid, gently stirred with a magnetic bar release gas. Take a rectangular test strip, at a distance of 4mm at a mark, with tweezers picked up the test paper, the mark soak the bottom of the short immersion agar, cooling, air drying for a few seconds. This repeated 2 to 4 times until the test paper to form a thickness of about 3mm agar plate layer. It is air-dried for 5 to 10 minutes and then stored in 50% ethanol solution. Before application, gently wipe the agar plate with a sterile swab or sponge. Retinal biopsy tissue specimens removed immediately placed on the agar; or specimens immersed in the Petri dish balanced solution,