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研究目的:探讨蒌叶(PB)提取物对5-氟尿嘧啶(5-FU)抑制结肠癌细胞HT29和HCT116生长的影响。研究方法:HT29和HCT116细胞分别给予PB、5-FU以及两种药物联合治疗24小时,应用等效线图法分析PB和5-FU的药效学相互作用,Annexin V/PI染色法检测HT29和HCT116细胞的凋亡情况,高效液相色谱法排除PB和5-FU间任何可能的相互化学作用。重要结论:联合PB,低剂量5-FU可以在短时间内起到细胞毒作用,而单独应用PB或5-FU治疗较联合治疗可以诱导更多细胞发生凋亡。进一步采用等效线图法分析显示PB和5-FU的联合作用在抑制结肠癌细胞HT29和HCT116的生长中分别体现出协同和拮抗作用。因此可以认为在HT29细胞中,PB使得较低剂量5-FU发挥最大抑制结肠癌细胞生长效果,然而在HCT116细胞中,PB没有显著降低5-FU的药物浓度,说明PB和5-FU的相互作用不仅仅体现在诱导细胞凋亡方面。
The purpose of this study was to investigate the effects of extract of leaves of PB on the growth of colon cancer cells HT29 and HCT116 induced by 5-fluorouracil (5-FU). Methods: HT29 and HCT116 cells were treated with PB, 5-FU and the two drugs for 24 hours respectively. The pharmacodynamic interaction between PB and 5-FU was analyzed by the isobologram method. The apoptosis of HT29 and HCT116 cells was detected by Annexin V / PI staining And HCT116 cell apoptosis, HPLC excludes any possible interaction between PB and 5-FU. Important conclusion: Combination of PB and low-dose 5-FU can induce cytotoxicity in a short time. However, PB or 5-FU alone can induce more cell apoptosis than combined treatment. Further analysis using the Equivalent Graph showed that the combined effects of PB and 5-FU showed synergistic and antagonistic effects in inhibiting the growth of colon cancer cells HT29 and HCT116, respectively. It is therefore believed that PB caused a maximum inhibition of colon cancer cell growth in lower doses of 5-FU in HT29 cells, whereas PBT did not significantly reduce the drug concentration of 5-FU in HCT116 cells, indicating that PB and 5-FU interact with each other The role is not only reflected in the induction of apoptosis.