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目的探讨葛根素预处理对局灶性脑缺血再灌注损伤的保护作用及其可能机制。方法健康成年雄性SD大鼠120只,随机分成5组(n=24):假手术组(S组),脑缺血再灌注组(IR组),Pc-24 h 100 mg组,Pc-24 h200 mg组,Pc-24 h400 mg组,其中Pc-24 h组于脑缺血前24 h给予相应剂量葛根素腹腔注射行单次预处理,采用大脑中动脉线栓法建立局灶性脑缺血再灌注模型,缺血90 min,再灌注24 h。大鼠清醒后进行神经功能缺陷评分,再灌注24 h时处死大鼠,处死后取脑组织,测定脑梗死容积比(BIVP)、脑组织含水量及MDA水平。结果葛根素预处理可以改善大鼠脑缺血再灌注损伤的神经功能缺损评分,降低BIVP、脑组织含水量及MDA水平(P均<0.01)。其中Pc-24 h 400 mg组减低神经功能缺损评分、BIVP及脑组织含水量明显优于Pc-24 h100 mg及Pc-24 h200 mg组(P均<0.01),而Pc-24 h100 mg及Pc-24 h200 mg组之间无显著差异(P均>0.05)。结论葛根素预处理可能通过抑制脑水肿及氧化损伤来减轻大鼠局灶性脑缺血再灌注损伤。葛根素预处理的脑保护作用具有一定的量效关系。
Objective To investigate the protective effect of puerarin preconditioning on focal cerebral ischemia-reperfusion injury and its possible mechanism. Methods One hundred and twenty healthy male Sprague-Dawley rats were randomly divided into 5 groups (n = 24): sham operation group (S group), cerebral ischemia reperfusion group (IR group) h200 mg group and Pc-24 h400 mg group. The Pc-24 h group was given a single pretreatment of the corresponding dose of puerarin intraperitoneally 24 h before cerebral ischemia, and focal cerebral ischemia was established by middle cerebral artery occlusion Blood reperfusion model, ischemia 90 min, reperfusion 24 h. The rats were scored for neurological deficits. After 24 hours of reperfusion, the rats were sacrificed and the brain tissues were sacrificed. The cerebral infarction volume ratio (BIVP), brain water content and MDA level were measured. Results Puerarin preconditioning could improve neurological deficit score, reduce BIVP, brain water content and MDA level (all P <0.01) in rats with cerebral ischemia-reperfusion injury. Pc-24 h 400 mg group decreased neurological deficit scores, BIVP and brain water content was significantly better than Pc-24 h100 mg and Pc-24 h200 mg group (P all <0.01), while Pc-24 h100 mg and Pc -24 h200 mg group no significant difference (P all> 0.05). Conclusion Puerarin preconditioning may reduce focal cerebral ischemia-reperfusion injury in rats by inhibiting brain edema and oxidative damage. Puerarin pretreatment of brain protection has a certain dose-effect relationship.