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目的探讨不同方法制备的金银花水提液对大鼠免疫功能的影响,为金银花的临床应用提供理论依据。方法分别采用水煎法和浸泡法制备金银花水提液,大鼠灌胃2周后,分别检测其巨噬细胞吞噬功能、淋巴细胞转化能力及TH1细胞分泌的IL-2、TNF-α、IFN-γ的mRNA表达情况。结果两种方法制备的金银花水提液均能改善机体的非特异性免疫功能,但只有水煎法制备的提取液能改善机体的细胞免疫功能。结论用水煎法制备金银花提取液改善机体免疫功能的功效优于浸泡法。
Objective To investigate the effects of aqueous extract of Lonicera Japonica L on the immune function of rats prepared by different methods and to provide a theoretical basis for the clinical application of honeysuckle. Methods Water extract and soaking were used to prepare the aqueous extract of honeysuckle. After 2 weeks of gavage, the phagocytosis of macrophages, the ability of lymphocyte transformation and the levels of IL-2, TNF-α and IFN secreted by TH1 cells -γ mRNA expression. Results The aqueous extract of honeysuckle prepared by the two methods could improve the non-specific immune function of the body, but only the extract prepared by decoction could improve the cellular immune function. Conclusion The method of decocting the honeysuckle extract to improve immune function is better than soaking method.