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美国Apollon公司报道了人类免疫缺陷症病毒(HIV)疫苗在临床前动物研究中的一项新的、极显著的结果.此疫苗是由病毒的两部分DNA序列组成,它能完全保护两只黑猩猩抵御大剂量HIV的攻击.Apollon公司的方法是以裸DNA接种为基础,将DNA质粒直接注入肌肉组织,细胞接受基因物质后产生由NDA编码的蛋白,注射病毒DNA后,随着病毒蛋白的产生,出现类似感染的效果,但不是真正的感染,无发病的危险.Apollon公司认为,此法主要特点是能刺激强烈的细胞毒性T细胞和抗体免疫应答.在猴实验中,Apollon公司和宾夕法尼亚大学的研究者研制了由两种DNA质粒组成的疫苗,其中一种质粒含编码HIV包膜蛋白gp160的基因和调节rev基因,另一种含编码核心蛋白p24的gag和pol基因.研究者用上述疫苗接种3只黑猩猩,另1只对照黑猩猩接受了非相关质粒.选取其中两只产生强免疫应答的动物,与对照动物一起用足够感染250只黑猩猩量的HIV攻击.攻击后数周接种动物血液中的病毒水平升高,但是没有进一步感染的迹象.于攻击后22周取淋巴结活检,用聚合酶链反应检测未见HIV征象.反之,对照动物在攻击后两周及其后的实验中出现明显感染.试验令人鼓舞的方面是,猴受到与疫苗株略有不同的HIV株的攻击,提示疫苗可能应付一定量的HIV突变倾向.
Apollon Corporation of the United States reported a new, highly significant result of a preclinical animal study of the human immunodeficiency virus (HIV) vaccine, which consists of a two-part DNA sequence of a virus that completely protects two chimpanzees Against the attack of large doses of HIV.Apollon’s method is based on naked DNA inoculation, the DNA plasmid injected directly into muscle tissue, cells receiving genetic material produced by the NDA-encoded protein, after injection of viral DNA, with the virus protein , A similar infection effect, but not a real infection, no risk of disease.Apollon believes that the main features of this method is to stimulate a strong immune response of cytotoxic T cells and antibodies.In the monkey experiment, Apollon Corporation and the University of Pennsylvania Researchers developed a vaccine consisting of two DNA plasmids, one of which contains the gene encoding the HIV envelope protein gp160 and the rev gene, the other containing the gag and pol genes that encode the core protein p24, Three chimpanzees were vaccinated and one control chimpanzee received unrelated plasmids Two of the animals that produced a strong immune response were selected for use with control animals Enough to infect 250 chimpanzees for HIV challenge.Viral levels in the blood of vaccinated animals increased for weeks after challenge but there was no sign of further infection.22 Lymph node biopsies were taken 22 weeks after challenge and no signs of HIV were detected by polymerase chain reaction Conversely, the control animals developed significant infections in the two weeks and beyond, and the encouraging aspect of the experiment was that the monkeys were attacked by HIV strains that were slightly different from the vaccine strain, suggesting that the vaccine may be able to cope with a certain amount of HIV Mutation tendency.