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目的:探讨建立不合并肾损害的尿结石模型。方法:100只雄性SD大鼠,随机分5组,空白对照组、化学纯三聚氰胺0.4～0.7 g.kg-1.d-1组,连续给药20,30 d,观察比较结石形成率、肾功能损害情况。结果:0.4,0.5 g.kg-1.d-1化学纯三聚氰胺给药20,30 d,膀胱结石率最高,20 d分别为60%,50%,30 d分别为50%,60%,两组及与0.6,0.7 g.kg-1.d-1组、空白对照组比较有显著性差异(P<0.05);膀胱结石形成率与剂量、时间不成线形关系;各组比较仅0.4 g.kg-1.d-1组肾和膀胱指数、肌酐、尿素氮、尿酸与空白对照组比较,无显著性差异。结论:化学纯三聚氰胺0.4 g.kg-1.d-1给药20 d对肾脏几乎不造成损害,SD大鼠膀胱结石率达60%,可建立不合并肾损害的尿结石模型。
Objective: To establish a urinary calculus model without renal damage. Methods: One hundred male Sprague-Dawley rats were randomly divided into five groups, blank control group and 0.4-0.7 g.kg-1.d-1 group. After continuous administration for 20 and 30 days, the incidence of stone formation, Functional impairment. Results: 0.4, 0.5 g.kg-1.d-1 melamine chemotherapy for 20,30 d, the highest rate of bladder stones, 20 d were 60%, 50%, 30 d were 50%, 60%, two Group and 0.6,0.7 g.kg-1.d-1 group, the blank control group were significantly different (P <0.05); bladder stone formation rate and dose and time is not linear relationship; each group only 0.4 g. kg-1.d-1 group kidney and bladder index, creatinine, urea nitrogen, uric acid compared with the blank control group, no significant difference. CONCLUSION: Nephrolithiasis induced by 0.4 g.kg-1.d-1 of chemical melamine for almost 20 days does not cause any damage to the kidneys and the volume of bladder stones in SD rats reaches 60%.