作者以48小时增殖抑制试验的体外方法研究了来自东非的12个恶性疟原虫分离株对氯喹、乙胺嘧啶和环氯胍的敏感性。所有种群先经连续体外培养适应后再传代培养1～2天,当原虫血症达0.3～1%时,取血细胞比积为5%的感染红细胞悬液加入预先加药板的各井中,培养48小时后作薄血膜检查。氯喹试验应用由WHO提供的标 The authors investigated the susceptibility of 12 Plasmodium falciparum isolates from East Africa to chloroquine, pyrimethamine and cycloguanidine in vitro in a 48-h proliferation inhibition assay. All populations were subcultured continuously for 1 to 2 days after in vitro culture adaptation. When protozoosis reached 0.3 to 1%, erythrocytes suspension with a hematocrit of 5% was added to each well of the pre-dosing plate and cultured After 48 hours for a thin blood film examination. Chloroquine test application provided by the WHO standard