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分别应用胶束电动毛细管色谱法(MECC)、反相色谱法和串联柱HPLC法分离了左旋十八甲基炔诺酮(ING)、睾酮(T)、孕酮(P)和雌二醇(E_2)等甾体化合物MECC分离在末涂层毛细管柱上进行.当电泳缓冲溶液为含20mmlo/L的2,6-甲基-β环糊精(DM-β-CD)添加剂和50mmol/L的十二烷基硫酸钠(SDS)的10mmol/L四硼酸钠(pH9.2)时,可实现快速分离.通过与反相色谱法和串联柱HPLC法相比较可以看出,几种甾体化合物的分离机理以疏水分配作用为主,缓冲液或固定相中的环糊精同时起着空间立体选择和疏水分配作用.环糊精的空腔大小和腔内取代基的疏水性均可影响分离的选择性.
(ING), testosterone (T), progesterone (P) and estradiol were separated by micellar electrokinetic capillary chromatography (MECC), reversed phase chromatography and tandem column HPLC E_2) and other steroid MECC separation on the capillary column.When electrophoresis buffer solution containing 20mmlo / L 2,6-methyl-β-cyclodextrin (DM-β-CD) additives and 50mmol / L Of sodium dodecyl sulfate (SDS) in 10mmol / L sodium tetraborate (pH9.2), rapid separation was achieved.Compared with reversed-phase chromatography and tandem column HPLC method, it can be seen that several steroid compounds Of the separation mechanism to the main role of the hydrophobic distribution, the buffer or stationary phase cyclodextrin also plays the role of three-dimensional spatial distribution and hydrophobic distribution of cyclodextrin cavity size and cavity hydrophobic substituent substituents can affect the separation The selectivity.