电泳技术的应用,历近50载,其方法不断发展,应用日趋广泛,遍及生物化学、分子生物学、细胞生物学和免疫化学等领域。尤以凝胶电泳,因其具有简便、快速、灵敏和高分辨等优点,已成为所有关系到蛋白质和核酸的实验室不可缺少的分析和制备手段。现就以下几个方面介绍凝胶电泳的新近进展。一、双向凝胶电泳(2-DGE) 朝两个方向分离蛋白质的电泳通常称为双向电泳。如平板电泳,在第一向电泳后旋 The application of electrophoresis technology, for nearly 50 years, its methods continue to develop, the increasingly widespread application, throughout the biochemistry, molecular biology, cell biology and immunochemistry and other fields. In particular, gel electrophoresis, because of its simplicity, speed, sensitivity and high resolution, has become an indispensable laboratory for all proteins and nucleic acids in the analysis and preparation. Now on the following aspects of gel electrophoresis recent progress. First, two-dimensional gel electrophoresis (2-DGE) to separate proteins in two directions electrophoresis is usually called two-dimensional electrophoresis. Such as plate electrophoresis, spin in the first run