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目的测定肺癌患者血清基质金属蛋白酶-9(MMP-9)、内皮抑素(endostatin,ES)、血管内皮生长因子(VEGF)水平,探讨其在肺癌中的特异性及其与化疗的关系。方法应用酶联免疫吸附法(ELISA)测定3种因子在144例肺癌患者血清中的表达。结果肺癌患者血清MMP-9、ES、VEGF分别为(678．06±38．79)ng/ml、(124．90±3．33) ng/ml及(809．81±129．43)pg/ml,显著高于对照组的(90．07±13．55)ng/ml、(61．0±5．15)ng/ml及(368．68±5．82)pg/ml,以及肺良性病变者的(228．96±15．39)ng/ml、(91．43±4．44)ng/ml及(490．14±14．58)pg/ml(P＜0．001)；肺癌患者血清MMP-9、ES及VEGF水平与肿瘤组织类型、有无远处转移、临床分期及原发肿瘤大小相关(P＜0．05)；化疗后3种因子分别为(569．23±100．08)ng/ml、(141．36±14．51)ng/ml及(680．94±130．47)pg/ml,与化疗前的(726．28±94．11)ng/ml、(127．86±13．24)ng/ml及(1415．01±587．22)pg/ml相比有显著性差异(P＜0．001)；肺癌患者血清MMP-9与ES水平呈正相关(r= 0．260．P=0．012)。结论肺癌患者血清MMP-9、ES、VEGF水平明显升高,在肺癌的病理疾病过程中有特殊意义,可作为预测肺癌恶性度和治疗反应的良好指标。
Objective To determine the serum levels of matrix metalloproteinase-9 (MMP-9), endostatin (ES) and vascular endothelial growth factor (VEGF) in patients with lung cancer and to investigate its specificity in lung cancer and its relationship with chemotherapy. Methods The serum levels of three factors in 144 lung cancer patients were determined by enzyme-linked immunosorbent assay (ELISA). Results The serum level of MMP-9, ES and VEGF in lung cancer patients were (678.06 ± 38.79) ng / ml, (124.90 ± 3.33) ng / ml and (809.81 ± 129.43) pg / ml, significantly higher than that of the control group (90.07 ± 13.55) ng / ml, (61.0 ± 5.15) ng / ml and (368.68 ± 5.82) pg / ml, (228.96 ± 15.39) ng / ml, (91.43 ± 4.44) ng / ml and (490.14 ± 14.58) pg / ml, respectively The levels of serum MMP-9, ES and VEGF were correlated with tumor type, distant metastasis, clinical stage and the size of primary tumor (P <0.05). The three factors after chemotherapy were (569.23 ± 100 (141.36 ± 14.51) ng / ml and (680.94 ± 130.47) pg / ml, respectively, which were significantly lower than those before chemotherapy (726.28 ± 94.11) ng / ml, (127.86 ± 13.24) ng / ml and (1415.01 ± 587.22) pg / ml, respectively (P <0.001). There was a positive correlation between serum MMP-9 and ES in patients with lung cancer (r = 0.260.P = 0.012). Conclusion Serum levels of MMP-9, ES and VEGF are significantly elevated in patients with lung cancer and have special significance in the pathological process of lung cancer, which may be used as a good indicator to predict the malignancy and response to treatment of lung cancer.