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以紫外光光刻、硅蚀刻及软光刻技术制备了微柱阵列型细胞培养基底。实验发现,在4μm的结构高度下,当微柱特征尺寸大于或等于4μm时,该法能制备结构规整清晰的聚二甲基硅氧烷微柱阵列型基底。特征尺寸为2μm的基底已经接近该法的极限加工能力,所加工的微柱阵列出现倒伏或缺失。采用一种简单的倾斜角法可以制备一种聚苯乙烯微球致密阵列型基底。该基底的微球直径可以小至2μm或亚微米尺度。细胞培养实验发现,拓扑结构基底能促进神经细胞粘附与铺展,影响细胞静息电位水平,增强电压依从式钙通道功能响应。阵列型拓扑结构基底为构建传感器细胞微环境提供了有效的工程化手段。
A micropillar array cell culture substrate was prepared by UV photolithography, silicon etching and soft lithography. It was found that the method can produce a structured and clear polydimethylsiloxane microcolumnarray substrate with a feature size of 4 μm or more at a structure height of 4 μm. Substrates with a feature size of 2 μm have approached the limit processing capability of the method, and the fabricated micro-pillars arrays appear to be lodging or missing. A simple tilt angle method can be used to prepare a dense array of polystyrene microspheres. The diameter of the microspheres of the substrate can be as small as 2 μm or submicron scale. Cell culture experiments showed that the topology of the substrate can promote nerve cell adhesion and spreading, affecting the level of cell resting potential and enhance voltage-dependent calcium channel function response. Array Topology Substrates provide an effective engineering tool for building sensor cell microenvironments.