论文部分内容阅读
目的通过探讨缺氧诱导因子1α(HIF-1α),靶基因血管内皮生长因子(VEGF)在胎儿生长受限(FGR)患者胎盘组织中的表达,研究FGR的发病机制。方法采用链酶菌抗生物素蛋白-过氧化物酶连接(SP)法检测FGR组和对照组的胎盘组织中的HIF-1α、VEGF的表达,并进行相关性分析。结果①FGR组胎盘组织中HIF-1α阳性表达率明显高于对照组,差异具有统计学意义(P<0.05)。②FGR组胎盘组织中VEGF阳性表达率低于对照组,两者比较,差异有统计学意义。③FGR组患者和对照组胎盘中HIF-1α表达与VEGF变化呈显著负相关(r=-0.622,P<0.05)。结论 HIF-1α在FGR患者胎盘组织中表达升高,通过下调靶基因VEGF,引起VEGF降低,影响胎盘血管重铸,参与胎儿生长受限的发生和发展。
Objective To investigate the pathogenesis of FGR by investigating the expression of hypoxia-inducible factor 1α (HIF-1α) and target gene VEGF (VEGF) in placenta of patients with fetal growth restriction (FGR). Methods The expression of HIF-1α and VEGF in placenta of FGR group and control group was detected by streptavidin avidin-peroxidase (SP) method and the correlation was analyzed. Results ① The positive rate of HIF-1α in placenta of FGR group was significantly higher than that in control group (P <0.05). ② The positive rate of VEGF expression in placenta of FGR group was lower than that in control group, the difference was statistically significant. ③ The expression of HIF-1α in placenta of patients with FGR and control group was negatively correlated with the change of VEGF (r = -0.622, P <0.05). Conclusion The expression of HIF-1α in placenta of FGR patients is increased. The downregulation of VEGF in target gene causes the decrease of VEGF, which affects the placental vascular remodeling and participates in the occurrence and development of fetal growth restriction.