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目的分析各T淋巴细胞亚群中CD147的表达水平以及CD147表达量在健康对照者外周血,乳腺癌患者外周血、淋巴结和肿瘤浸润淋巴细胞中CD4+T细胞的变化,分析CD147对乳腺癌患者预后的影响。方法采用Robust Multi-array Averaging的标准化方法提取GEO芯片GSE14308、GSE36765和GSE6532中的CD147表达数据。不同的基因表达水平的比较采用方差分析,生存曲线采用Log-Rank检验以及Cox比例风险回归模型进行多因素分析。结果 CD147在C57BL/6J小鼠淋巴结中的Th1细胞、Th2细胞、Th17细胞表达水平是Th0细胞的8~12倍。在乳腺癌患者的CD4+肿瘤浸润淋巴细胞中,CD147的表达水平显著高于健康对照者外周血、乳腺癌患者外周血和淋巴结中的CD4+T细胞。Log-Rank分析中,CD147表达量高低与无复发生存时间显著相关。Cox多元回归模型显示CD147高表达是乳腺癌复发的危险因素(风险度=1.764;95%可信区间:1.088~2.859)。结论 CD147可能通过上调CD4+T细胞的功能参与炎症反应,促进乳腺癌的进展。
Objective To analyze the expression of CD147 in various T lymphocyte subsets and the expression of CD147 in peripheral blood of healthy controls, the changes of CD4 + T cells in peripheral blood lymph nodes and tumor infiltrating lymphocytes in patients with breast cancer. The impact of prognosis. Methods CD147 expression data of GEO chip GSE14308, GSE36765 and GSE6532 were extracted by Robust Multi-array Averaging. Comparison of different gene expression levels using analysis of variance, survival curves using Log-Rank test and Cox proportional hazards regression model for multivariate analysis. Results The expression of CD147 in Th1 cells, Th2 cells and Th17 cells in lymph nodes of C57BL / 6J mice was 8 ~ 12 times higher than that of Th0 cells. The expression of CD147 in CD4 + tumor infiltrating lymphocytes of breast cancer patients was significantly higher than that in healthy controls, CD4 + T cells in peripheral blood and lymph nodes of breast cancer patients. In Log-Rank analysis, the level of CD147 expression was significantly correlated with the recurrence-free survival time. Cox multivariate regression models showed that CD147 overexpression was a risk factor for breast cancer recurrence (risk = 1.764; 95% confidence interval: 1.088-2.859). Conclusion CD147 may be involved in the inflammatory response by up-regulating the function of CD4 + T cells and promoting the progression of breast cancer.