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目的:探讨树突状细胞(S 100)、抑癌基因p63、金属蛋白酶组织抑制因子 1(TIMP 1)在喉癌组织中 的表达及与吸烟、饮酒、病理学分级、临床TNM分期和预后等因素的关系。方法:应用组织芯片技术和免疫组织 化学SP法检测85例喉鳞状细胞癌组织中S 100、p63、TIMP 1等因子的表达。结果:85例中,6例组织芯片脱落 或无肿瘤细胞。对79例进行S 100检测,其中47例(59.5%)出现阳性细胞(0～34个/视野)。S 100阳性树突状 细胞浸润程度与喉癌分化程度、临床分期差异均有统计学意义(均P<0.05)。对79例进行TIMP 1检测,其中 35例(44.3%)为阴性表达,44例(55.7%)为阳性表达。TIMP 1蛋白阳性表达与患者肿瘤临床分期、有无淋巴结 转移及预后差异均有统计学意义(均P<0.05)。在79例中,58例p63表达阳性(73.4%),平均染色比率为 45.5%,但未发现与临床因素相关。结论:TIMP 1、S 100与肿瘤的生长、分化有关,可作为临床检测预后的重要 参考指标。
Objective: To investigate the expression of dendritic cells (S 100), tumor suppressor gene p63 and tissue inhibitor of metalloproteinase 1 (TIMP-1) in laryngeal carcinoma and their association with smoking, drinking, pathological grade, TNM staging and prognosis The relationship between factors. Methods: The expression of S 100, p63 and TIMP-1 in 85 cases of laryngeal squamous cell carcinoma were detected by tissue microarray and immunohistochemistry SP method. Results: In 85 cases, 6 cases of tissue chip shedding or no tumor cells. S 100 was detected in 79 patients, of which 47 (59.5%) showed positive cells (0 ~ 34 per field). S 100 positive dendritic cell infiltration and laryngeal cancer differentiation, clinical stage differences were statistically significant (P <0.05). TIMP-1 was detected in 79 cases, of which 35 (44.3%) were negative and 44 (55.7%) were positive. The positive expression of TIMP-1 was significantly correlated with the tumor stage, lymph node metastasis and prognosis (all P <0.05). In 79 cases, 58 cases of p63 expression was positive (73.4%), the average staining rate was 45.5%, but no correlation with clinical factors was found. Conclusion: The expressions of TIMP-1 and S-100 are related to tumor growth and differentiation, which may be used as an important reference for clinical detection of prognosis.