目的获得高纯度的重组单纯疱疹病毒Ⅰ型HSV-1糖蛋白BgB胞浆区蛋白并制备其特异性抗体。方法从感染HSV-1的BHK细胞中提取总RNA用RT-PCR特异性扩增HSV-1gB胞浆区编码基因,经双酶切后,克隆入表达载体pGEX4T-1中,进行融合表达。以纯化的重组蛋白免疫小鼠制备抗体,进行Westernblot鉴定。结果用IPTG诱导后,表达出相对分子质量Mr约42000的融合蛋白。用纯化的融合蛋白免疫小鼠制备的抗体滴度为1400。结论获得重组HSV-1gB胞浆区蛋白及其特异性抗体,为后续功能研究奠定了基础。 Objective To obtain high purity recombinant herpes simplex virus type  HSV-1 glycoprotein BgB cytoplasmic protein and preparation of its specific antibodies. Methods The total RNA was extracted from BHK cells infected with HSV-1. The coding region of HSV-1gB was amplified by RT-PCR and cloned into the expression vector pGEX4T-1 . Antibodies were prepared by immunizing mice with purified recombinant protein and identified by Western blot. Results After induction with IPTG, a fusion protein with a relative molecular mass of about 42,000 was expressed. The antibody titer prepared by immunizing mice with the purified fusion protein was 1  400. Conclusion Obtaining the recombinant protein of HSV-1gB and its specific antibody, which laid the foundation for the follow-up study of function.