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目的:观察李氏5号中药对D-半乳糖老化小鼠海马超微结构和磷脂酰肌醇酯3激酶(phosphatidylinoditide-3’OHkinase,PI3K)、胰岛素受体底物-1(insulin-receptorsubstrate-1,IRS-1)、胰岛素受体底物-2(insulinreceptorsubstrate-2,IRS-2)表达的影响。方法:将132只小鼠随机分为6组,正常对照(C)组、D-半乳糖模型(D)组、李氏5号大剂量(L)组、中剂量(M)组、小剂量(S)组、多奈哌齐(A)组。D,L,M,S,A5组每日皮下注射半乳糖100mg/kg。L,M,S3个治疗组分别灌胃李氏5号方1.2,0.6,0.3g/(kg·d),A组多奈哌齐0.75mg/(kg·d)灌胃。4个月后,取小鼠海马CA1区组织进行电镜观察,行脑冷冻切片,用PI3K,IRS-1,IRS-2抗体进行免疫组化染色。结果:①电镜结构显示,D-半乳糖老化小鼠海马CA1区神经元核固缩,线粒体、高尔基体等细胞器肿胀,血管足突细胞肿胀,管腔变窄;李氏5号中药保护可以明显改善这种现象。②免疫组织化学染色结果显示3种抗体C组深染的阳性细胞在海马广泛分布,D组海马阳性反应细胞着色浅,少有突起,细胞数目少,分别为C组的48.8%,57.0%和60.8%。李氏5号方组染色结果与C组相似,A组亦有相同结果。结论:D-半乳糖老化小鼠海马CA1区出现明显超微结构病理改变,海马PI3K,IRS-1,IRS-2的表达明显下降;李氏5号中药保护能明显改善CA1区?
OBJECTIVE: To observe the ultrastructural and phosphatidylinoditide-3’ OHkinase (PI3K) and insulin-receptor substrate-1 of hippocampus in aging mice induced by D-galactose. 1, IRS-1), Insulin receptor substrate-2 (IRS-2) expression. METHODS: 132 mice were randomly divided into 6 groups: normal control (C) group, D-galactose model (D) group, Lee’s 5 high dose (L) group, medium dose (M) group, and low dose (S) group, donepezil (A) group. In groups D, L, M, S, and A5, galactose 100 mg/kg was subcutaneously injected daily. The L, M, and S treatment groups were intragastrically administered with Lee 5’s 1.2, 0.6, and 0.3 g/(kg·d) respectively, and the group A was treated with donepezil 0.75 mg/(kg·d). Four months later, the hippocampal CA1 region of the mouse was taken for electron microscopy, brain frozen sections were performed, and PI3K, IRS-1, and IRS-2 antibodies were used for immunohistochemical staining. RESULTS: 1 Electron microscopy revealed that the neurons in the hippocampal CA1 region of D-galactose-aged mice were nucleus pyknosis, mitochondria, Golgi apparatus and other organelles were swollen, and the vascular foot process cells were swollen and the lumen was narrowed; the protection of Lee’s No. 5 Chinese medicine was obvious. Improve this phenomenon. 2The results of immunohistochemical staining showed that the positive cells in group C were deeply distributed in the hippocampus. The positive cells in hippocampus in group D were stained lightly with few protrusions and the number of cells was small, which was 48.8%, 57.0% of that in group C, respectively. 60.8%. The staining results of the Lee’s No. 5 group were similar to those of the C group, and the A group had the same results. Conclusion: The pathological changes of hippocampal CA1 region were obviously observed in D-galactose-induced aging mice. The expression of PI3K, IRS-1 and IRS-2 in hippocampus was significantly decreased. The protective ability of Lishi No.5 Chinese herbs significantly improved the CA1 region.