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目的观察海马突触体外周型苯二氮受体(PBR)水平对大鼠空间学习和记忆能力的影响。方法SD大鼠随机分为对照组和D-半乳糖组。D-半乳糖组动物连续皮下注射D-半乳糖(100mg/kg,每日1次)56次,对照组注射生理盐水。利用Morris水迷宫测试动物学习记忆能力后制备海马突触体,测定PBR最大结合容量(Bmax)和平衡解离常数(KD)。结果与对照组比较,D-半乳糖组动物学习记忆能力降低(P<0.001),KD无显著变化。D-半乳糖组Bmax(177.2fmol/mg±26·7fmol/mg)低于对照组(296.7fmol/mg±33.5fmol/mg)(P<0.001),海马突触体[3H]PK11195特异性结合活性与迷宫试验动物逃避潜伏期(r=-0.854)、平台象限游泳时间(r=0.845)及距离(r=0·851)显著相关(P<0.001)。结论海马突触体PBR表达量可能与大鼠空间学习和记忆有关。
Objective To observe the effects of peripheral hippocampal synapse receptors (PBR) on spatial learning and memory in rats. Methods SD rats were randomly divided into control group and D-galactose group. D-galactose group animals were injected subcutaneously with D-galactose (100mg / kg once daily) for 56 times. The control group was injected with normal saline. The hippocampal synaptosomes were prepared by Morris water maze test, and the maximal PBR and KD of PBR were measured. Results Compared with the control group, the ability of learning and memory of D-galactose group was decreased (P <0.001), and KD had no significant change. Bmax in the D-galactose group (177.2 fmol / mg ± 26.7 fmol / mg) was lower than that in the control group (296.7 fmol / mg ± 33.5 fmol / mg) (P <0.001) There was significant correlation between activity and labyrinth latency (r = -0.854), platform quadrant swimming time (r = 0.845) and distance (r = 0.851) (P <0.001). Conclusion The expression of PBR in hippocampal synaptosome may be related to spatial learning and memory in rats.