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Rho GTP激酶对调控细胞骨架具有重要影响,其可控制微丝、微管的生长与维持,进而对细胞形态发生、细胞迁移、内吞转运等生命活动产生影响。已知细胞对纳米药物的摄取离不开细胞骨架的调控作用,但已有研究却很少关注Rho GTP激酶对细胞摄取纳米药物或纳米药物递送载体的影响。为了探究这一问题,本研究选择了单壁碳纳米角作为纳米药物或纳米药物递送载体的一种模型,其独特的物理化学性质使其成为药物递送领域热门研究对象。利用激光共聚焦显微镜和TEM观察细胞对纳米材料的摄取情况以及胞内分布;随后以各类内吞抑制剂处理细胞,分析细胞对单壁碳纳米角的摄取机制。实验结果表明,Rho GTP激酶中的Rho A被抑制后会显著减少细胞对单壁碳纳米角的摄取。单壁碳纳米角经网格蛋白介导的内吞入胞,入胞后主要分布在溶酶体中。
Rho GTP kinase plays an important role in the regulation of cytoskeleton, which can control the growth and maintenance of microfilaments and microtubules, and then affect the life activities such as cell morphogenesis, cell migration and endocytosis. It is known that the uptake of nanomedicine by cells is inseparable from the regulation of the cytoskeleton. However, few studies have focused on the effect of Rho GTP kinase on the cellular uptake of nanomedicine or nanomedicine delivery carriers. In order to explore this issue, we selected single-wall carbon nanohorns as a model for nanomedicine or nanomedicine delivery carriers, and its unique physicochemical properties make it a hot research target in drug delivery. Using laser confocal microscopy and TEM to observe the cell uptake of nanomaterials and intracellular distribution; then treated with various types of endocytosis inhibitors, analyzed the cell uptake mechanism of single-walled carbon nanohorns. The experimental results show that the inhibition of Rho A in Rho GTP kinase can significantly reduce the cell uptake of single-walled carbon nanohorns. Single-walled carbon nanohorn relies on clathrin-mediated endocytosis of cells, mainly distributed in lysosomes.