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为探讨CD15与胃癌发生发展和转移的关系,我们应用免疫组化法检测了胃癌组织中CD15抗原表达,并进一步用胶体金免疫电镜技术对CD15在大肠癌细胞内的分布特征进行了观察。 1 材料和方法 1.1 材料 胃癌95例为我院外科手术切除标本,其中男73例,女22例,平均发病年龄59.8岁,标本经100mL/L甲醛固定,石蜡包埋、切片。对部分新鲜胃癌标本切成0.5cm×0.5cm的小组织块,放入40mL/L多聚甲醛—戊二醛固定,鼠抗CD15单抗、胶体金标记二抗(金颗粒15nm)和LSAB试剂盒为DAKO
To investigate the relationship between CD15 and the development, metastasis of gastric cancer, we used immunohistochemistry to detect the expression of CD15 antigen in gastric cancer tissues, and further examined the distribution characteristics of CD15 in colorectal cancer cells by colloidal gold immunoelectron microscopy. 1 Materials and methods 1.1 Materials Ninety-five cases of gastric cancer were surgical specimens removed from our hospital, including 73 males and 22 females. The average age of onset was 59.8 years. Specimens were fixed in 100 mL/L formalin, paraffin-embedded and sectioned. A piece of fresh tissue of gastric cancer was cut into 0.5cm×0.5cm small tissue pieces and placed in 40mL/L paraformaldehyde-glutaraldehyde. The mouse anti-CD15 monoclonal antibody, colloidal gold labeled secondary antibody (gold particle 15nm) and LSAB reagent Box for DAKO