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静脉注射内毒素后4h,狗肝脏Ⅱ型蛋白激酶A活力显著低于对照组,对酶激活物CAMP以及酶反应底物ATP和组蛋白的最大反应速度显著降低,米氏常数无变化;狗肝脏Ⅰ型蛋白激酶A活力、最大反应速度及米氏常数均无变化。结果提示,狗内毒素血症时肝脏Ⅱ型蛋白激酶A活性受抑。
4h after intravenous injection of endotoxin, the activity of type II PKA in dog liver was significantly lower than that in control group, the maximal reaction speed to enzyme activator CAMP and enzyme reaction substrates ATP and histone was significantly reduced, but the Mick’s constant did not change; Type I protein kinase A activity, the maximum reaction rate and the Michaelis constant no change. The results suggest that hepatic type II PKA activity is inhibited in dogs with endotoxemia.