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目的探讨巢式PCR法检测急性淋巴细胞白血病（ALL）微小残留细胞（MRD）在评价疗效、预测复发、判断预后的意义。方法对28例ALL的患儿采用巢式PCR法扩增T细胞受体TCR的Vδ2─Dδ3重排片段，检测急淋患儿的骨髓（BM）及外周血（PB）标本。结果在完全缓解（CR）后一段时间内仍有MRD存在，随访8例中，CR后MRD存在但渐少者呈持续缓解，而CR后MRD持续阳性或由阴转阳，提示复发机会增大。结论巢式PCR检测急淋缓解期MRD对预测复发、指导治疗有重要意义。
Objective To explore the significance of nested-PCR in detection of acute residual lymphocytic leukemia (ALL) minimal residual cells (MRD) in evaluating efficacy, predicting recurrence, and predicting prognosis. Methods Nested PCR was used to amplify the Vδ2-Dδ3 rearrangement of TCR TCR in 28 children with ALL. The bone marrow (BM) and peripheral blood (PB) specimens were detected in children with acute leukemia. Results MRD was still present in the period of time after complete remission (CR). In the follow-up of 8 cases, MRD was present but gradually decreased after CR, and MRD continued to be positive or changed from negative to positive after CR, suggesting an increased chance of relapse. . Conclusion Nested-PCR detection of acute lymphoblastic MRD is important for predicting recurrence and guiding treatment.