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目的:检测结直肠癌中氨肽酶N(APN)的表达,并通过分析其与血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的关系,探讨APN在结直肠癌发生、发展过程中的作用及其临床意义。方法:应用免疫组织化学技术,检测40例结直肠癌组织及40例癌旁正常直肠组织中APN、VEGF和bFGF的表达,并结合临床病例特征进行分析。结果:40例结肠癌组织中APN的阳性率达52.5%(21/40),15例转移淋巴组织中达66.67%(10/15),在结直肠癌组织和转移淋巴结组织中的阳性表达率均明显高于癌旁正常直肠组织,差异有统计学意义(P<0.05)。APN、VEGF与bFGF在结直肠癌中的阳性表达均与患者的性别、年龄、肿瘤大小、肿瘤位置、分化程度无关(P>0.05),而与肿瘤的分期和有无转移显著相关(P<0.05)。结直肠癌组织中APN与VEGF、bFGF的表达间均呈显著正相关性(P<0.05)。结论:APN在结直肠癌组织中呈高表达,与VEGF、bFGF可能具有协同作用,共同参与调节结直肠癌的发展,对于评估结直肠癌的生物学行为和预测患者的预后可能具有重要价值。
OBJECTIVE: To detect the expression of aminopeptidase N (APN) in colorectal cancer and to explore the relationship between the expression of APN and colorectal cancer by analyzing its relationship with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) , The role of development and its clinical significance. Methods: The expressions of APN, VEGF and bFGF in 40 colorectal cancer tissues and 40 adjacent normal rectal tissues were detected by immunohistochemistry. The clinical features were analyzed. Results: The positive rate of APN in 40 colon cancer tissues was 52.5% (21/40), and that in 15 metastatic lymphoid tissues was 66.67% (10/15). The positive rate of APN in colorectal cancer tissues and metastatic lymph nodes tissues Were significantly higher than the adjacent normal rectal tissue, the difference was statistically significant (P <0.05). The positive expression of APN, VEGF and bFGF in colorectal cancer were not related with the gender, age, tumor size, tumor location and differentiation (P> 0.05), but significantly correlated with tumor stage and metastasis (P < 0.05). The expression of APN, VEGF and bFGF in colorectal cancer showed a significant positive correlation (P <0.05). CONCLUSION: APN is highly expressed in colorectal cancer tissues. It may play a synergistic role with VEGF and bFGF in regulating the development of colorectal cancer. It may be of great value in assessing the biological behavior of colorectal cancer and predicting the prognosis of patients.