氯霉素(chloramphenicol,CAP)由于具有较强的毒副作用,欧盟规定禁止在食用动物中使用,我国也明令禁止将其用作兽药,并要求所有动物可食组织中不得检出。在目前所有的分析方法中,液相色谱-串联质谱法(LC-MS/MS)因其灵敏度高、无须衍生化等特点而成为氯霉素残留的主要测试方法,我国食品安全国家标准中对牛奶中氯霉素残留量的测定也采用LC-MS/MS方法[1],样品处理需用乙酸乙酯提取,再用C18柱净化。本文简化了样品前处理步骤,样品不需溶剂提取,经冷冻离心后,直接用Chem Elut柱净化,乙酸乙酯洗脱后经正己烷脱脂,同位素稀释后 Chloramphenicol (CAP) due to its strong side effects, the European Union banned the use of food animals, China also banned its use as a veterinary drug, and requires that all animal edible tissue can not be detected. Among all the current analytical methods, LC-MS / MS is the main method for the determination of chloramphenicol residues due to its high sensitivity and without derivatization. In our country, the national standard for food safety The determination of chloramphenicol residues in milk was also performed by LC-MS / MS method [1]. Samples were processed with ethyl acetate and cleaned with C18 column. This article simplifies the sample preparation steps, the sample without solvent extraction, after centrifugation by centrifugation, directly with Chem Elut column purification, ethyl acetate eluting after n-hexane, isotope dilution