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目的:检测Hep G2细胞中CYP3A、SLCO1B1、POR的单核苷酸多态性(SNP)位点CYP3A4*1G、CYP3A4*22、CYP3AP1*3、CYP3A5*3、SLCO1B1 T521C、SLCO1B1 A388G、POR*28的基因型。方法:从Hep G2细胞中提取基因组DNA,PCR扩增目的片段,对PCR扩增产物测序后分析上述7个多态性位点的基因型。结果与结论:CYP3A4*1G、CYP3A4*22、CYP3AP1*3、CYP3A5*3、SLCO1B1 T521C、SLCO1B1 A388G、POR*28的基因型分别为GA、CC、GA、AG、TC、AG、CC。CYP3A4*1G下游596 bp处基因型为CT,SLCO1B1 T521C下游50 bp和76 bp处基因型分别为TC和CT,POR*28下游281 bp处基因型为GA。
AIM: To detect single nucleotide polymorphisms (SNPs) of CYP3A, SLCO1B1 and POR in Hep G2 cells, including CYP3A4 * 1G, CYP3A4 * 22, CYP3AP1 * 3, CYP3A5 * 3, SLCO1B1 T521C, SLCO1B1 A388G, POR * 28 Genotype. Methods: The genomic DNA was extracted from Hep G2 cells, and the target fragment was amplified by PCR. The genotypes of the seven polymorphic loci were analyzed after PCR amplification products were sequenced. RESULTS AND CONCLUSION: The genotypes of CYP3A4 * 1G, CYP3A4 * 22, CYP3AP1 * 3, CYP3A5 * 3, SLCO1B1 T521C, SLCO1B1 A388G and POR * 28 were GA, CC, GA, AG, TC, AG and CC respectively. The genotypes at 596 bp downstream of CYP3A4 * 1G were CT, the genotypes at 50 bp and 76 bp downstream of SLCO1B1 T521C were TC and CT respectively, and the genotype at 281 bp downstream of POR * 28 was GA.