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目的:探讨尿酸对类成骨细胞(MG-63)增殖的影响及其可能机制。方法:将生长状态良好的类成骨细胞(MG-63)分为四组,分别为对照组(加入成骨培养液的完全培养基)和实验组(分别加入成骨培养液及含0.2、0.4、0.8mmol/L尿酸的完全培养基),诱导第14天,在倒置显微镜下观察细胞形态变化,分别在第7天和第14天检测类成骨细胞(MG-63)碱性磷酸酶活性,CCK-8法检测细胞增殖情况以及RT-PCR法检测TGF-β1 mRNA的表达。结果:尿酸干预类成骨细胞(MG-63)后,细胞数目随着尿酸浓度的升高逐渐增加,以0.8 mmol/L最明显。类成骨细胞(MG-63)碱性磷酸酶活性与增殖能力均增高;细胞TGF-β1 mRNA表达升高,呈现浓度、时间依赖性;以上指标于实验组与对照组间以及各实验组组间比较,差异均有统计学意义(P<0.05)。结论:尿酸可刺激类成骨细胞(MG-63)增殖,可能与促进TGF-β1转录有关。
Objective: To investigate the effect of uric acid on the proliferation of osteoblasts (MG-63) and its possible mechanism. Methods: The osteoblasts (MG-63) with good growth status were divided into four groups: control group (complete medium added with osteogenic medium) and experimental group (osteoblast culture medium containing 0.2, 0.4, 0.8 mmol / L uric acid). On the 14th day of induction, morphological changes of the cells were observed under an inverted microscope. At the 7th day and the 14th day, the osteoblast (MG-63) alkaline phosphatase Activity, CCK-8 assay of cell proliferation and RT-PCR assay TGF-β1 mRNA expression. RESULTS: After urate-induced osteoblasts (MG-63), the number of cells increased gradually with increasing uric acid concentration, most notably at 0.8 mmol / L. The alkaline phosphatase activity and proliferation ability of osteoblasts (MG-63) were increased. The expression of TGF-β1 mRNA was increased in a concentration-and time-dependent manner. The above indexes were significantly different between the experimental group and the control group Between the two groups, the differences were statistically significant (P <0.05). Conclusion: Uric acid can stimulate the proliferation of osteoblasts (MG-63), which may be related to the promotion of TGF-β1 transcription.