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目的:研究微小RNA-34a(microRNA-34a,miR-34a)在人脑胶质瘤组织中的表达以及其对胶质瘤SHG-44细胞增殖和凋亡的影响。方法:20例胶质瘤组织标本均取自于青岛医学院附属医院神经外科(2007年01月至2010年12月),作为对照的正常脑组织取自5位重症脑外伤需行减压手术的患者。Real-time PCR检测胶质瘤组织中miR-34a的表达。体外转染miR-34a mimics至SHG-44细胞中,MTT实验、流式细胞术检测SHG-44细胞的增殖、细胞周期及凋亡。结果:miR-34a在人脑胶质瘤组织中的表达量明显低于正常脑组织,其在Ⅲ、Ⅳ期胶质瘤组织中表达量明显低于Ⅰ、Ⅱ期胶质瘤组织。miR-34amimics体外转染组与空白组相比,其细胞增殖抑制率明显提高[(37.24±5.72)%vs(4.19±0.63)%,P<0.01];miR-34amimics转染组SHG-44细胞G1期比例明显高于空白对照组[(61.78±2.01)%vs(50.91±1.19)%,P<0.05];且miR-34a转染组细胞凋亡率与空白组细胞相比显著升高[(15.28±3.65)%,vs(2.07±0.84)%,P<0.01]。结论:miR-34a在人脑胶质瘤组织中低表达,miR-34a可抑制SHG-44细胞的增殖、诱导细胞周期阻滞和细胞凋亡。
AIM: To investigate the expression of microRNA-34a (miR-34a) in human glioma tissue and its effect on the proliferation and apoptosis of glioma SHG-44 cells. Methods: Twenty cases of glioma tissues were obtained from Department of Neurosurgery, Affiliated Hospital of Qingdao Medical College (January 2007 to December 2010). The normal brain tissue taken from 5 patients with severe traumatic brain injury underwent decompression surgery Of patients. Real-time PCR was used to detect the expression of miR-34a in glioma tissues. MiR-34a mimics was transfected into SHG-44 cells in vitro. The proliferation, cell cycle and apoptosis of SHG-44 cells were detected by MTT assay and flow cytometry. Results: The expression of miR-34a in human glioma tissues was significantly lower than that in normal brain tissues. The expression of miR-34a in stage Ⅲ and Ⅳ glioma tissues was significantly lower than that in stage Ⅰ and Ⅱ gliomas. The inhibitory rate of miR-34amimics in vitro transfection group was significantly higher than that of blank group [(37.24 ± 5.72)% vs (4.19 ± 0.63)%, P <0.01]; miR-34amimics transfected SHG-44 cells G1 phase was significantly higher than that in the blank control group [(61.78 ± 2.01)% vs (50.91 ± 1.19)%, P <0.05], and the apoptosis rate in miR-34a transfected group was significantly higher than that in the blank group [ (15.28 ± 3.65)%, vs (2.07 ± 0.84)%, P <0.01]. Conclusion: miR-34a is down-regulated in human glioma tissues. MiR-34a can inhibit the proliferation of SHG-44 cells and induce cell cycle arrest and apoptosis.