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对新疆芥菜型油菜4个单、双低品系子叶柄再生频率的影响因素进行了研究,其中子叶柄日龄、6-BA、AgNO3、卡那霉素浓度及基因型等因素对不定芽的再生率有很大影响。试验结果表明:萌发4~5d的幼苗子叶柄再生频率最高;6-BA、AgNO3。对不定芽分化的促进效果在不同品系中有较大差异。通常浓度为4~6mg/L;不同品系对卡那霉素的耐受力有很大不同,其中新95-A在Kan15mg/L时仍有4.17%的绿苗率;再生芽生根较容易,移栽成活率较高。
The factors affecting the regeneration frequency of four petiole and two low strains of Xinjiang Brassica napus were studied, including the regeneration of adventitious buds, including the age of cotyledonary petiole, 6-BA, AgNO3, kanamycin concentration and genotype Rate has a great impact. The results showed that the regeneration frequency of seedling petiole was the highest between 4 and 5 days after germination; 6-BA and AgNO3. The promotion of adventitious bud differentiation in different strains are quite different. Usually 4 ~ 6mg / L; different strains of kanamycin tolerance are very different, including the new 95-A Kan15mg / L still 4.17% of the green seedling rate; Easy, transplanting survival rate higher.