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目的:探讨金丝桃素单体、苦味叶下珠水溶液浸出物、茵陈水溶性提取物体外抗人巨细胞病毒(HCMVAD169)的作用。方法:采用细胞病变(CPE)抑制法观察金丝桃素单体、苦味叶下珠水溶液浸出物、茵陈水溶性提取物对HCMV AD169毒株的抑制作用,使用MTT比色法检测细胞的损伤程度,并利用吸光度(A)值评价这三种中药抗HCMV的效应。结果:金丝桃素单体、苦味叶下珠水溶液浸出物、茵陈水溶性提取物半数中毒浓度(TC50)分别为195.96,310.57,904.49 mg/L;半数有效浓度(IC50)分别为19.15,51.25,195.11 mg/L;治疗指数(TI)分别为10.23,6.06,4.64;IC50下,对HCMV的抑制率分别为47.6%,41.7%,42%。细胞培养96 h,IC50下,光学倒置相差显微镜下:金丝桃素单体抑制组中约30%贴壁细胞变成圆型或发生肿胀,而另外两种中药抑制组中约45%-55%贴壁细胞变成圆型或发生肿胀。金丝桃素单体、苦味叶下珠水溶液浸出物、茵陈水溶性提取物溶液分别在6.25,10,3.2 mg/L浓度下具有显著的抗HCMV的作用,且随药物浓度的增高其作用而增强。苦味叶下珠水溶液浸出物和茵陈水溶性提取物抗HCMV作用的差异无显著性(P>0.05),而金丝桃素单体与其他两种中药抗HCMV作用的差异有显著性(P<0.05)。结论:金丝桃素单体、苦味叶下珠水溶液浸出物及茵陈水溶性提取物均是理想的抗HC-MV中药,其中金丝桃素单体抗HCMV的作用尤为突出。
OBJECTIVE: To investigate the effects of hypericin (HCMVAD169) against hypericin in vitro in vitro by hypericin, extract of Aphis citricola and aqueous extract of Achillea purpurea. Methods: Inhibition of cytopathic effect (CPE) was used to observe the inhibitory effect of hypericin, extract of Phyllanthus urinaria and aqueous extract of Rhizoma etongcum on HCMV AD169 strain. MTT assay was used to detect cell injury Degree, and the absorbance (A) value of the evaluation of these three Chinese medicine anti-HCMV effect. Results: The concentrations of hypericin, Phyllanthus urinaria and Leucoma pubescens were 195.96, 310.57 and 904.49 mg / L, respectively. The half effective concentrations (IC50) were 19.15, 51.25 and 195.11 mg / L, respectively. The therapeutic indexes (TI) were 10.23, 6.06 and 4.64, respectively. The inhibitory rates for HCMV were 47.6%, 41.7% and 42%, respectively. Cell culture 96 h, IC50, optical inverted phase contrast microscope: hypericin monomer inhibition group about 30% of adherent cells into a round or swollen, while the other two types of Chinese medicine inhibition group about 45% -55 % Adherent cells become round or swollen. Hypericin monomer, aqueous extract of bitter leaf beads aqueous solution, Yin Chan water-soluble extract solution at 6.25,10,3.2 mg / L concentrations have a significant anti-HCMV role, and with the drug concentration increased its role And enhanced. There was no significant difference in the anti-HCMV effect between the extracts of Aqueous extract of Bitter Leaf Phyllanthus and Rhizoma et Radix Aconiti Lateralis (P> 0.05), while there was a significant difference between the hypericin and other two herbs in anti-HCMV activity (P <0.05). CONCLUSION: Hypericin, Acacia pubescens aqueous extract and Acanthopanax senticosus aqueous extracts are both ideal anti-HC-MV herbs. Hypericin is particularly effective against HCMV.