“我们必须打破常规,尽量采用先进技术”。在中国科学院遗传研究所403组的协助下,我们进行了甲基磺酸乙酯对水稻诱变效果的试验,现把结果简报如后: 一、材料和方法供引变品种:“科字5—4”、“湘矮早九号”。均用单系种子,保证有高度纯一性。浸种处理方法:先将种子预浸6小时,晾干后浸入药液中,置于25℃温度下。药液用PH=7、浓度为0.01m的磷酸缓冲液作溶剂,将EMS分别配成0.3％、O.4％、O.5％和0.6％四种,以单用缓冲液浸种作对照。浸泡时间分为12小时和24小时。浸种24小时的,在浸后12小时换新药一次。处理完后在流水中漂洗24小时,重新燥干,10天后播种。 “We must break the routine and try to adopt advanced technology.” With the assistance of 403 groups of Institute of Genetics, Chinese Academy of Sciences, we conducted a test of the effect of ethyl methanesulfonate on rice mutagenesis. The results are briefly presented as follows: I. Materials and Methods For the lead varieties: “Section 5 -4 ”,“ Xiang short morning on the 9th. ” Single seeds are used to ensure a high degree of purity. Soaking seed treatment methods: The seeds are pre-soaked 6 hours, immersed in liquid medicine after drying, placed at a temperature of 25 ℃. The solution was prepared with pH = 7 and 0.01 M phosphate buffer as the solvent, and the EMSs were respectively formulated into 0.3%, 0.4%, 0.5% and 0.6% of four solutions. Soaking time is divided into 12 hours and 24 hours. Soak for 24 hours, 12 hours after immersion in the new drug once. Rinsing in running water for 24 hours after treatment, drying again, sowing after 10 days.