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对4周龄雌性(C57BL/6×DBA/2)F_1(BDF_1)小鼠,静脉投与亲系6周龄雌性C57BL/6小鼠的脾细胞混悬液(4×10~7cells/mouse),每隔6日1次,投与3次,制成急性GVHR(移植物抗宿主反应)模型。与制作急性GVHR模型一样,对BDF_1小鼠静脉投与亲系7周龄雌性DBA/2小鼠的脾细胞混悬液(4×10~7cells/mouse),投与3次,制成慢性GVHR模型。实验用小鼠以10只为1组。将人参养荣汤、当归芍药散、
For 4-week-old female (C57BL/6×DBA/2)F_1 (BDF_1) mice, spleen cell suspension (4×10~7 cells/mouse) of 6-week old female C57BL/6 mice was intravenously administered. The acute GVHR (graft-host response) model was prepared by administering it once every 6 days. Similarly to the production of an acute GVHR model, a suspension of spleen cells (4×10~7 cells/mouse) of a 7-week old female DBA/2 mouse was intravenously administered to BDF_1 mice and administered three times to prepare a chronic GVHR model. The experimental mice consisted of 10 mice as a group. The ginseng culture Rong Tang, Danggui Shaoyao Powder,