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目的:比较桃儿七、小叶莲化学成分的异同,分析桃儿七、小叶莲的化学成分与毒性的关系,为建立鬼臼毒素类藏药的指纹图谱提供依据。方法:利用HPLC分析方法,分析桃儿七、小叶莲化学成分的差异,结合急性毒性实验方法测定桃儿七和小叶莲的LD50值,比较两药毒性差异,分析桃儿七、小叶莲鬼臼成分与毒性的关系,为桃儿七、小叶莲的质量标准提供依据。结果:标定了桃儿七与小叶莲的14个共有峰;桃儿七药材中鬼臼毒素、4’-去甲鬼臼毒素含量明显高于小叶莲,桃儿七、小叶莲LD50分别为0.088 7,0.909 8 g·mL-1。结论:桃儿七、小叶莲化学成分基本相同,但化学成分含量差异较大。桃儿七鬼臼毒素含量更高,毒性更强,在制定药材质量标准时应严格控制鬼臼毒性成分的含量。
OBJECTIVE: To compare the chemical constituents of the peach seven and the small lotus leaf, analyze the relationship between the chemical constituents and the toxicity of the peach seven and the small lotus leaf lotus, and provide the basis for the establishment of the fingerprints of the podophyllotoxin type Tibetan medicine. Methods: The HPLC method was used to analyze the differences of the chemical constituents of Taoyiqi and Xiaoyunshen. The LD50 values of Taoyuqi and Xiaoyunshen were determined with acute toxicity test. The differences of toxicity between the two drugs were compared. Composition and toxicity of the relationship between the quality of Taoyu seven leaf lotus provide the basis. Results: The 14 common peaks of Taoyuqi and Xiaoyu were calibrated. The content of podophyllotoxin and 4’-demethoxypodophyllotoxin in Shijiazhuang seven herbs was significantly higher than that of Xiaoyu lotus, Taohe seven, and Xiaoyunshan respectively 7,0.909 8 g · mL-1. Conclusion: The chemical constituents of Taoyuqi and Xiaoyunshan are basically the same, but the content of chemical components is quite different. Peach podophyllotoxin content higher, more toxic, in the development of medicinal quality standards should be strictly controlled podophyllotoxin content.