目的:探索乳杆菌肽聚糖免疫调节作用的机制。方法:BALB/c小鼠腹腔注射乳杆菌肽聚糖,从腹腔巨噬细胞和脾淋巴细胞提取RNA,基因芯片分析基因表达情况,基于已知的基因网络数据库利用PathwayExplorer和GeneMAPP分析乳杆菌肽聚糖刺激特异性的基因网络。结果:PathwayEx-plorer分析得到的最显著变化的基因网络是“细胞因子-细胞因子受体相互作用”和“辅助性T细胞表面分子”。GeneMAPP分析得到最显著基因网络是核糖体蛋白、炎性反应基因网络和血红素合成基因网络。结论:乳杆菌肽聚糖刺激引起大量蛋白表达,引起保护性炎性反应,激活Th细胞,可能引起Th1免疫反应。 Objective: To explore the mechanism of immune regulation of Lactobacillus peptidoglycan. METHODS: BALB / c mice were injected intraperitoneally with Lactobacillus peptidoglycan, RNA was extracted from peritoneal macrophages and splenic lymphocytes, gene expression was analyzed by microarray, and based on known gene network databases, Pathway Explorer and GeneMAPP were used to analyze Lactobacillus polygalacturonase Sugar stimulates specific gene networks. RESULTS: The most significant changes in gene network analyzed by PathwayEx-plorer were “cytokine-cytokine receptor interaction” and “helper T-cell surface molecule”. GeneMAPP analysis of the most significant gene network is the ribosomal protein, inflammatory response gene network and heme synthesis gene network. Conclusion: Lactobacillus peptidoglycan stimulated a large number of protein expression, causing a protective inflammatory reaction, activating Th cells, may cause Th1 immune response.