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目的探讨用RT-PCR技术诊断小儿肠道病毒性脑炎的临床应用价值。方法选取我院2008-06～2009-08期间诊断为病毒性脑炎的住院患儿。于入院的当天抽取脑脊液,经RT-PCR反应,筛选出肠道病毒病例,结果用SPSS13.0进行统计。结果共有45例标本检测出肠道病毒(EV),其中,男20例,检出率76.9%;女25例,检出率83.3%;男女间差异无显著性,(P>0.05)。8月～3岁组16例,检出率80%;3～6岁组18例,检出率81.8%;6～12岁11例,检出率78.6%;各组间差异无显著性,(P>0.05)。EV感染病例与阳性对照可扩增出单一相同的电泳条带;所有阴性对照病例均无扩增。整个检测过程平均用时4.6h。结论①RT-PCR技术可以快速做出病原学诊断,且敏感性高,无非特异性扩增。②该方法操作简单,值得临床上进一步推广。③我院2008-06-2009-08期间诊断为病毒性脑炎的住院患儿以肠道病毒感染为主。④肠道病毒在小儿中为普遍易感,未见有性别及年龄差异性。
Objective To investigate the clinical value of RT-PCR in the diagnosis of children’s enteroviral encephalitis. Methods The hospitalized children diagnosed with viral encephalitis during our hospital from June 2008 to June 2009 were selected. Cerebrospinal fluid (CSF) was drawn on the day of admission, and enterovirus was screened out by RT-PCR. The results were analyzed by SPSS13.0. Results A total of 45 specimens were detected for enterovirus (EV). Among them, 20 were male and the detection rate was 76.9%. There were 25 women with the detection rate of 83.3%. There was no significant difference between men and women (P> 0.05). The detection rate was 80% in August ~ 3 years old group, 18 cases in 3 ~ 6 years old group, the detection rate was 81.8%; in 11 cases of 6 ~ 12 years old, the detection rate was 78.6% (P> 0.05). EV infection cases and positive control can amplify a single identical electrophoresis bands; all negative control cases were no amplification. The entire testing process takes an average of 4.6h. Conclusion ① RT-PCR can make rapid etiological diagnosis, and high sensitivity, non-specific amplification. ② The method is simple, it is worth to further promote the clinical. ③ hospital in our hospital during 2008-06-2009-08 diagnosis of viral encephalitis in children with enterovirus infection. ④ enterovirus in children is generally susceptible, no gender and age differences were seen.