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采用PCR-变性梯度凝胶电泳技术(PCR-DGGE)分析比较了34株分离于环境和水产养殖动物体内的副溶血弧菌及标准株16S-23S rDNA间区(Intergenic Spacer Region,ISR)的多态性和亲缘关系。结果表明,34株副溶血弧菌的ISR经PCR-DGGE电泳后均能分离出4―10条条带,共计产生15个多态性位点。所有菌株聚为H、I、J、K四大簇,株间遗传差异最大为株A18和A25,遗传距离达到0.4。ISR PCR-DGGE方法为副溶血弧菌基因分型提供了一种新的方法。
The PCR-DGGE analysis of 34 isolates of V. parahaemolyticus and 16S-23S rDNA Intergenic Spacer Region (ISR) isolated from environment and aquaculture animals Status and kinship. The results showed that all the 34 isolates of V. parahaemolyticus could isolate 4-10 bands after PCR-DGGE electrophoresis, and a total of 15 polymorphic loci were generated. All strains were clustered into H, I, J, K four clusters, the largest genetic differences between strains A18 and A25, the genetic distance of 0.4. The ISR PCR-DGGE method provides a new method for genotyping Vibrio parahaemolyticus.