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利用反相高效液相色谱法定量分析米曲霉菌种培养基中３硝基丙酸的含量。样品经乙酸乙酯提取、净化后，以 Ｈｙｐｅｒｓｉｌ Ｃ１８为色谱柱，以 Ｃ Ｈ３ Ｃ Ｎ／ Ｋ Ｈ２ Ｐ Ｏ４ （００２ ｍ ｏｌ／ Ｌ，ｐ Ｈ３０）（１／３，ｖ／ｖ）为流动相，流速为１０ｍ ｌ／ｍ ｉｎ，紫外检测器波长２１０ｎｍ 。该方法最低检出量为１０μｇ／ｋｇ（信噪比为３），回收率为９０１％ ～９８０％ 。
The content of 3-nitropropionic acid in Aspergillus oryzae culture medium was quantitatively determined by RP-HPLC. The sample was extracted with ethyl acetate and purified. The sample was separated on a Hypersil C18 column with C H3 C N / K H 2 PO 4 (002 m ol / L, p H 3 O) (1/3, v / v) As mobile phase at a flow rate of 1 0m l / m in, UV detector wavelength 210nm. The detection limit of the method was 10μg / kg (signal to noise ratio of 3), the recovery rate was 901% ~ 980%.