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AIM: To study the blockade of paeoniflorin (Pae) on I_(Na) in the acutely isolated hippocampus neurons of mice. METHODS: The whole-cell patch clamp technique was used. RESULTS: Pae inhibited I_(Na) in frequency-dependent and concentration-dependent manners, with an IC_(50) of 271μmol/L. Pae 0.3 mmol/L shifted the activation potential of the maximal I_(Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I_(Na) inactivation state from (4.2±0.7) ms to (9.8±1.2) ms. CONCLUSION: Pae inhibited I_(Na) in mouse hippocampus neurons.
AIM: To study the blockade of paeoniflorin (Pae) on I_ (Na) in the acutely isolated hippocampus neurons of mice. METHODS: Pae inhibited I_ (Na) in frequency-dependent and concentration-dependent manners, with an IC 50 of 271 μmol / L. Pae 0.3 mmol / L shifted the activation potential of the maximal I- (Na) from -40 mV to -30 mV, shifted the steady-state activation and inactivation curves toward more positive and negative potentials by 10.8 mV, and 18.2 mV, respectively, and postponed the recovery of I_ (Na) inactivation state from 4.2 ± 0.7 ms to 9.8 ± 1.2 ms. CONCLUSION: Pae inhibited I_ (Na) in mouse hippocampus neurons.