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目的 应用壳聚糖神经导引管作为神经再生室桥接大鼠坐骨神经缺损 ,观察对神经再生的作用。方法 选用体重 2 0 0± 2 0g的Wistar大鼠 30只 ,手术造成右侧坐骨神经长约 12mm的缺损 ,以壳聚糖导管桥接神经缺损 ,以左侧正常坐骨神经作为对照 ,分别于术后 4、 12、 2 4周进行大体及显微解剖观察、组织学检查、免疫组化检查、电镜观察和神经电生理测定。结果 术后大鼠右后肢感觉、运动功能有不同程度的恢复 ;光镜和电镜组织学检查发现术后 12周再生轴突已长过神经缺损间隙 ,2 4周再生完全 ,髓鞘化良好 ,神经纤维排列整齐规则 ,导管大部分被降解吸收 ;神经电生理检查在术后 2 4周记录到再生坐骨神经的复合动作电位。结论 壳聚糖神经导引管为大鼠坐骨神经再生提供一个良好的再生微环境 ,再生坐骨神经功能恢复良好
OBJECTIVE: To observe the effect of chitosan neurotransduction on bridging sciatic nerve defects in rats with nerve regeneration compartment. Methods Thirty Wistar rats weighing 200 ± 20 g were selected. The defect of the right sciatic nerve about 12 mm in length was made. The defect of the defect was bridged with chitosan and the left normal sciatic nerve was taken as the control. At 12, 24 weeks, gross and microscopic anatomical observation, histological examination, immunohistochemistry, electron microscopy and electrophysiological examination were performed. Results The sensory and motor functions of the right hindlimb in rats recovered to varying degrees. The histological examination of the axons at 12 weeks after operation revealed that the axons of the regenerative axons had been longer than the gap of the nerve defects, completely regenerated at 24 weeks, and had good myelination, Nerve fibers arranged neat rules, most of the catheter is degraded and absorbed; Electrophysiological examination 24 weeks after surgery recorded the compound action potential of the regenerative sciatic nerve. Conclusion Chitosan nerve guide tube provides a good regenerative microenvironment for the regeneration of sciatic nerve in rats and the function of regenerative sciatic nerve recovered well